t7-expression vector pet14b Search Results


bamhi  (New England Biolabs)
99
New England Biolabs bamhi
Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet14b  (Millipore)
90
Millipore pet14b
Pet14b, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pet14b - by Bioz Stars, 2026-03
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pet14b t7 expression vector  (Millipore)
90
Millipore pet14b t7 expression vector
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
Pet14b T7 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pet14b t7 expression vector/product/Millipore
Average 90 stars, based on 1 article reviews
pet14b t7 expression vector - by Bioz Stars, 2026-03
90/100 stars
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pet-28a  (Millipore)
90
Millipore pet-28a
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
Pet 28a, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pet-28a - by Bioz Stars, 2026-03
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t4 dna ligase  (New England Biolabs)
99
New England Biolabs t4 dna ligase
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
t4 dna ligase - by Bioz Stars, 2026-03
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fastdigest bamhi  (Thermo Fisher)
90
Thermo Fisher fastdigest bamhi
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
Fastdigest Bamhi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fastdigest bamhi - by Bioz Stars, 2026-03
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escherichia coli bl21(de3)rosetta cells  (Millipore)
90
Millipore escherichia coli bl21(de3)rosetta cells
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
Escherichia Coli Bl21(De3)Rosetta Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s-adenosylmethionine synthetase  (Molecular Biosciences Inc)
90
Molecular Biosciences Inc s-adenosylmethionine synthetase
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
S Adenosylmethionine Synthetase, supplied by Molecular Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgem7  (Promega)
90
Promega pgem7
( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with <t>pET14b</t> vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.
Pgem7, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgem7/product/Promega
Average 90 stars, based on 1 article reviews
pgem7 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with pET14b vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.

Journal: PLoS ONE

Article Title: B-Cell Epitopes in GroEL of Francisella tularensis

doi: 10.1371/journal.pone.0099847

Figure Lengend Snippet: ( A ) ELISA (data from one of two experiments with similar results are shown). Lysates of E. coli BL21 transformed with pET14b vector containing Ft SchuS4 GroEL DNA, Ft LVS GroEL DNA, or no insert (empty vector) were coated on ELISA plates in the left panels, and greater than 10-fold higher concentrations of SchuS4, LVS, or E. coli TG1 lysates were coated on ELISA plates in the right panels. The coated plates were probed with serial dilutions of the indicated mAbs. ( B ) Western blot. Equivalent concentrations of lysates (based on OD 600 of the bacterial cultures) of SchuS4 or of BL21 transformed with SchuS4 rGroEL-vector or empty vector were electrophoresed in preparative 4–15% polyacrylamide gels under denaturing conditions and, after transfer to nitrocellulose, strips were probed with 10 µg/ml of the indicated mAbs. The positions of prestained molecular weight standards (in kDa) are indicated.

Article Snippet: The NdeI/XhoI-cleaved PCR product was initially cloned into a PCR 2.1-TOPO vector (Life Technologies, Grand Island, NY) and then transferred into the pET14b T7 expression vector (Novagen, Madison, WI) to add DNA encoding an N-terminal sequence of six histidine residues (a His tag) to the GroEL gene. pET14b (empty vector) and pET14b containing the SchuS4 GroEL gene insert or the LVS GroEL gene insert were separately transformed into One Shot BL21(DE3)pLysS chemically competent E. coli (Life Technologies), and induction of protein expression was carried out in LB broth for 4 hours at 37°C in the presence of IPTG (isopropyl-β-d-thiogalactopyranoside) according to manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay, Plasmid Preparation, Western Blot, Molecular Weight